Improved phosphate biosorption by bacterial surface display of phosphate-binding protein utilizing ice nucleation protein
نویسندگان
چکیده
منابع مشابه
Cell surface display of organophosphorus hydrolase using ice nucleation protein.
A new anchor system based on the ice nucleation protein (InaV) from Pseudomonas syringae INA5 was developed for cell surface display of functional organophosphorus hydrolase (OPH). The activity and stability of cells expressing the truncated InaV (INPNC)-OPH fusions were compared to cells with surface-expressed OPH using two other fusion anchors based on Lpp-OmpA and the truncated InaK protein....
متن کاملIdentification and purification of a bacterial ice-nucleation protein.
The protein product of a gene (inaZ) responsible for ice nucleation by Pseudomonas syringae S203 has been identified and purified after overexpression in Escherichia coli. The amino acid composition and the N-terminal sequence of the purified, denatured protein corresponded well with that predicted from the sequence of the inaZ gene. The product of inaZ was also found to be the major component ...
متن کاملFunctional analysis of glycin rich- RNA binding protein, a suppressor of trehalose-6-phosphate mediating growth arrest in Arabidopsis thaliana
Metabolism of the alpha-1,1 glucose disaccharide, trehalose, is indispensable in plants. In the Murashigeand Skoog (MS) medium, trehalose inhibits plant growth and allocation of carbon to roots. A suppressorof trehalose-6-phosphate (T6P) mediated growth arrest, GR-RBP2, is characterized in more detail.Phylogenetic analysis revealed that GR-RBP2 is a protein of likely prokaryot...
متن کاملPhosphate binding sites identification in protein structures
Nearly half of known protein structures interact with phosphate-containing ligands, such as nucleotides and other cofactors. Many methods have been developed for the identification of metal ions-binding sites and some for bigger ligands such as carbohydrates, but none is yet available for the prediction of phosphate-binding sites. Here we describe Pfinder, a method that predicts binding sites f...
متن کاملRestoration of phosphate transport by the phosphate-binding protein in spheroplasts of Escherichia coli.
Reconstitution of phosphate transport in Escherichia coli was demonstrated. Conversion of E. coli K10 cells to spheroplasts decreased phosphate transport to about 2%. Addition of purified phosphate-binding protein at physiological levels to these spheroplasts caused a mean 14-fold increase in phosphate transport rate. Crude shock fluid fractions were also stimulatory but not if the shock fluid ...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 2009
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.2009.01724.x